FISH-mapped CEPH YACs spanning 0 to 46 cM on human chromosome 6

Journal Article · Thu Aug 15 04:00:00 EDT 1996 · Genomics

DOI:https://doi.org/10.1006/geno.1996.0430· OSTI ID:484330

Bray-Ward, P; Bowlus, C; Choi, J ^[1]

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1. Yale Univ. School of Medicine, New Haven, CT (United States); and others

Seventy-six CEPH YACs were mapped by fluorescence in situ hybridization (FISH) to human metaphase chromosomes. These clones have been ordered from pter to 46 cM by combining the results of FISH with sequence-tagged site content mapping using data from the public databases. This created a minimal tiling path containing at least 37 Mb of human genomic DNA from 0 to 46 cM on chromosome 6 that contains up to four gaps not greater than 200 kb. These data provide an integration of the FLpter physical map values with cytogenetic band localization and markers on the genetic and radiation hybrid maps. We also assessed YAC chimerism and placed three additional Whitehead contigs within the integrated map. 27 refs., 1 fig., 1 tab.

• OSTI ID: 484330
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 1 Vol. 36; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
BANDING TECHNIQUES
BIOLOGICAL MARKERS
CHIMERAS
CHROMOSOMAL ABERRATIONS
CONTIGS
DATA BASE MANAGEMENT
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
FLUORESCENCE
GENES
GENETIC MAPPING
HEREDITARY DISEASES
HISTOCOMPATIBILITY COMPLEX
HUMAN CHROMOSOME 6
MAN
POLYMERASE CHAIN REACTION