Progress towards complete genetic and physical maps of chromosome 3
- Univ. of Texas Health Science Center, San Antonio, TX (United States); and others
Human chromosome 3 contains 210 million base pairs and approximately 7,000 genes. Our center is directed towards making genetic and physical maps of chromosome 3 with the goal of a YAC contig of the chromosome and 2500 STSs. Markers are first binned into 23 regions of chromosome 3 using framework somatic cell hybrids. To date, STSs for 259 microsatellite and 63 genes have been placed into bins. 125 polymorphic simple sequences repeat (SSR) markers were genetically mapped to chromosome 3 using PCR-based genotyping of the CEPH reference families. This genetic map spans 271 cM (sex averaged). A chromosome 3-specific radiation-reduced somatic cell hybrid panels has been constructed to obtain higher resolution map order information. With the map locations of these markers as a guide, 226 loci were screened using PCR-based hierarchial screening of the CEPH Mark I and Mark IV-VII yeast artificial chromosome (YAC) libraries. YAC contigs which are ordered on the chromosome consist of 33 multi-locus contigs covering 80 cM and single locus contigs accounting for 62 cM. We have isolated approximately 1000 YACs by this method covering 65% of chromosome 3. IRS-PCR screening of the CEPH YAC library has yielded several thousand more chromosome 3 YACs which are being screened with the STSs to integrate them into contigs. FISH analysis on a random sampling of 60 YACs from the long arm was performed to assess the degree of chimerism. 40% of the YACs hybridized to the map locations predicted by the STSs used in their isolation. 30% were obviously chimeric while 40% hybridized uniquely to the predicted region as well as nonspecifically to centromeres and telomeres. This integrated approach to constructing a map of chromosome 3 will result in a reliably ordered YAC contig for this chromosome.
- OSTI ID:
- 134452
- Report Number(s):
- CONF-941009--
- Journal Information:
- American Journal of Human Genetics, Journal Name: American Journal of Human Genetics Journal Issue: Suppl.3 Vol. 55; ISSN AJHGAG; ISSN 0002-9297
- Country of Publication:
- United States
- Language:
- English
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