DNA labelling in bone marrow cells of X-irradiated mice receiving homologous ³²P-lymphocytes

Journal Article · Tue Aug 01 00:00:00 EDT 1961 · Experimental Cell Research

DOI:https://doi.org/10.1016/0014-4827(61)90441-4· OSTI ID:4843126

Hill, M.

Labelled lymphocytes obtained from thymuses of donor mice injected with inorganic P³² were given to recipient mice subjected to x-irradiation with 800 r 24 hr previously. The DNA labeling of recipients' bone marrow cells was studied in Feulgen-stained autoradiographs 2 and 6 hrs after the treatment. The highest frequency of labelling was found in granulocytes which were followed in a descending order by mononuclear cells, reticular cells, megakaryocytes, and myelocytes. Mitotic figures were not labelled. The label was localized either evenly all over the nucleus or unevenly in certain areas of the nucleus. It is suggested that the recipients' bone marrow cells were labelled by taking up DNA-P³² from disintegrating donor lymphocytes and by incorporating it into their nuclei.

• Research Organization: Inst. of Biophysics, Czechoslovak Academy of Sciences, Brno
• Sponsoring Organization: USDOE
• NSA Number: NSA-15-029022
• OSTI ID: 4843126
• Journal Information: Experimental Cell Research, Journal Name: Experimental Cell Research Journal Issue: 2 Vol. 24; ISSN 0014-4827
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ANIMAL CELLS
BIOLOGY AND MEDICINE
BONE MARROW
CYTOLOGY
LYMPHOCYTES
MICE
MITOSIS
NUCLEIC ACIDS
PHOSPHORUS 32
RADIATION DOSES
THYMUS
TRACER TECHNIQUES
X RADIATION