THE METABOLISM OF CARBON-14-LABELLED COMPOUNDS BY STAPHYLOCOCCUS AUREUS
Technical Report
·
OSTI ID:4836325
The carbon precursors of the polynucleotide purines and pyrimidines were determined for a strain of S. aureus by the addition of C/sup 14/-labeled nutrients added singly to a medium of known constitution. The results obtained were consistent with the precursors found for the purines of the pigeon, the rat, yeast, and other bacteria. Glycine, formate, and bicarbonate were found to be contributors of carbon, though only in the case of glycine were the specific activities obtained high enough to be unequivocal. Glucose, serine, and alanine also contributed carbon presumably via a prior conversion to CO/sub 2/, "1-C" units, and glycine, though here too the specific activities were very low. As in other organisms, aspartate was found to be the principal precursor of the nucleic acid pyrimidines. Bicarbonate glycine, and formate were also carbon contributors, though the specific activities obtained were not large enough to be unequivocal in meaning. The strain of S. aureus studied was found to be able to utilize exogenously supplied uracil for nucleic acid uracil synthesis and was found to be able to transform it into the other polynucleotide pyrimidines. Thymine was not utilized at all. Cytosine was utilized only slightly for polynucleotide cytosine synthesis. Exogenously available guanine and adenine could both be used for polynucleotide purine synthesis. However, while guanine could be converted into polynucleotide adenine, the reverse conversion took place only to a small extent. Both purines had an effect of the incorporation of the other purine when present in greater amount. Both purines were able to depress de novo purine synthesis. (auth)
- Research Organization:
- Pennsylvania. Univ., Philadelphia
- NSA Number:
- NSA-15-031958
- OSTI ID:
- 4836325
- Report Number(s):
- TID-13351
- Country of Publication:
- United States
- Language:
- English
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