Preparation and Purificaton of Human Insulin-I131; Binding to Human Insulinbinding Antibodies
Journal Article
·
· Journal of Clinical Investigation
The preparation of I131-labeled human insulin from a lot of human insulin containing approximately 25% insulin by weight and its purification from labeled contaminants are described. The reaction of human insulin-I133 with insulinbinding antibodies in the serums of human subjects treated with commercial mixtures of animal insulins is demonstrated directly. Comparison of the binding of human insulin and beef insulin in antiserums from eight insulinresistant and nonresistant diabetic subjects revealed a lesser affinity' of antibody for human than for beef insulin in most cases, but considerable variability in this respect was encountered among different antiserums.
- Research Organization:
- Veterans Administration Hospital, Bronx, N.Y.
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-15-031967
- OSTI ID:
- 4836252
- Journal Information:
- Journal of Clinical Investigation, Journal Name: Journal of Clinical Investigation Journal Issue: 10 Vol. 40; ISSN 0021-9738
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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