Inactivation of Urease by Nitrogen Mustard and a Comparison with Inactivation by X-rays
Journal Article
·
· International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine
Urease is inactivated pry small amounts of nitrogen mustard (Di-2- (chloroethyl) methylamine; HN2) in unbuffered solution at pH 7.0, the inhibition or inactivation being complete when 10-3 M nitrogen mustard is used. With 10-5 mI solutions of HN2, 12% inhibition was observed. The inhihitnon is pH-dependent, the percentage inactivation with 10-3 M HN2 being 100 at pH 6.5 and 0 at pH 5.2. Additions of many different substances protected the urease. Buffer salts protected, citrate and phosphate being neost effective; for example, urease was completely protected against inactivation by 10-3 M nitrogen mustard by the addition of 5 x 10-3 mI, or stronger, solutions of citrate buffer. Proteins are effective protectors, but nucleic acids are not. AlI the amino acids tested protect, but cysteine and histidine are much more effective than most others investigated. Blocking of urease - SH groups is also an effective protective measure. The inactivation is considered likely to be a resuIt of the combination of nitrogen mustard with histidine residues and with - SH groups of the urease.
- Research Organization:
- St. Bartholomew's Hospital, London
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-16-001531
- OSTI ID:
- 4834986
- Journal Information:
- International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine, Journal Name: International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine Journal Issue: 6 Vol. 3; ISSN 0020-7616
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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