DEVELOPMENT OF TRANSPLANTATION ISOANTIGENS IN THE MOUSE EMBRYO AND TROPHOBLAST
Technical Report
·
OSTI ID:4827682
Female LAF/sub 1/ mice were mated with BALB/c males, and the pregnancies were surgically interrupted at various times of gestation. The embryos were extirpated, homogenized in Tyrode's solution, and equal quantities were injected subcutaneously into the mother and into a paired virgin LAF/sub 1/ female. One week following surgery and/or injection the mice were exposed to a lethal dose of whole-body x radiation (870 rad). Within 2 hours, each received an intravenous injection of 6 -- 9 x 10/sup 6/ bone marrow cells derived from male BALB/c mice. The presence of paternal transplantation immunity antigens in the injected fetal tissue was in dicated by death (within 21 day postirradiation) of the recipient mice, due to bone marrow rejection. Transplantation immunity antigens were detected in the developing mouse embryo as early as the 1 day pregnancy embrgo. Twenty-one day mortalities following the injection or fetal tissue into nonpregnant female mice were as follows: 1st trimester--39%; 2nd trimester--81%; 3rd trimester --98%. The data also show that the pregnant mother is to some extent immunologically non-reactive, since the injection or 1st trimester fetal tissue in this case elicited no 21-day mortality (0 out of 22). However, when 2nd and 3rd trimester fetal tissue was injected into the pregnant mothers, 21-day mortality was 66% and 82%, respectively. Therefore the maternal tolerance of the embryo seems best explained by the presence of an immunologically inert barrier of trophoblastic cells. (auth)
- Research Organization:
- Naval Radiological Defense Lab., San Francisco
- NSA Number:
- NSA-16-021917
- OSTI ID:
- 4827682
- Report Number(s):
- USNRDL-TR-556
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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