SOME EFFECTS OF IONIZING RADIATIONS ON THE ALKALINE PHOSPHATASE IN COW'S MILK
The effects of ionizing radiation on the alkaline phosphatase activity of milk were studied under different environmental conditions. Twenty-five megarads were required to inactivate the enzyme completely. A combination of heat treatment and irradiation given either before or after heating showed that inactivation of the enzyme was greater when heating occurred after irradiation, this effect of the different forms of energy possibly indicating different mechanisms of heat inactivation. Varying the dose rate over the range 12-250 krad/min did not affect the amount of enzyme inactivated. After irradiation, activity declined during storage at 30 deg C but not at 5 deg C. Irradiation did not change the value of K/sub m/ yet the V/sub max/ fell, indicating that there are fewer active sites present after irradiation but that affinity of the remaining sites for the substrate remains unchanged. (Public Health Eng. Abstr. 42: No. 7, July 1962)
- Research Organization:
- Wantage Research Lab., Berks, Eng.
- NSA Number:
- NSA-16-026806
- OSTI ID:
- 4821350
- Report Number(s):
- AERE-R-3363
- Journal Information:
- J. Dairy Research, Journal Name: J. Dairy Research Vol. Vol: 29: No. 1
- Country of Publication:
- United Kingdom
- Language:
- English
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