THE UNCOUPLING OF OXIDATIVE PHOSPHORYLATION BY IONIZING RADIATION

Journal Article · Wed Aug 01 04:00:00 EDT 1962 · Can. J. Biochem. and Physiol.

DOI:https://doi.org/10.1139/o62-115· OSTI ID:4808473

Scaife, J F; Hill, B

Whole-body irradiation of rabbits or rats with x rays or Co/sup 60/ gamma rays causes uncoupling of oxidative phosphorylation in thymus mitochondria, which is not prevented by the prior administration of AET. Whole- body irradiation was not found to affect oxidative phosphorylation in liver or mouse ascites cell mitochondria. The radiation lesion can be repaired in vitro by the addition of cytochrome c, bovine serum albumin, or vitamin K/sub 1/ to mitochondria. Vitamin E and coenzyme Q/sub 10/ were without effect. Both phosphorylating steps in the electron transport chain associated with succinate oxidation are affected by irradiation. The diphosphopyridine nucleotide dependent steps in the oxidation of alpha -ketoglutarate by thymus mitochondria are damaged by in vivo irradiation. Diphosphopyridine nucleotide levels of thymus and spleen but not liver or ascites cells are reduced by in vivo irradiation. No effect of in vitro irradiation on oxidative phosphorylation could be found for thymocyte cell suspensions, isolated thymus or liver mitochondria, or ascites or HeLa cell suspensions. Respiration of ascites or thymocyte cells was unaffected by in vitro irradiation. (auth)

• Research Organization: Defence Research Chemical Labs., Ottawa
• Sponsoring Organization: USDOE
• NSA Number: NSA-16-028774
• OSTI ID: 4808473
• Journal Information: Can. J. Biochem. and Physiol., Journal Name: Can. J. Biochem. and Physiol. Vol. Vol: 40
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

AET
ALBUMINS
ANIMAL CELLS
BIOCHEMISTRY
BIOLOGY AND MEDICINE
BLOOD SERUM
BODY
CATTLE
COBALT 60
CYTOCHROMES
DRUGS
ENZYMES
GAMMA RADIATION
LIVER
MITOCHONDRIA
ORGANIC SULFUR COMPOUNDS
OXIDATION
RABBITS
RADIATION EFFECTS
RADIATION INJURIES
RADIATION PROTECTION
RATS
THYMUS
VITAMIN E
VITAMIN K
VITAMINS
X RADIATION