PHOSPHATE PARTITION IN THE ERYTHROCYTES OF NORMAL NEWBORN INFANTS AND INFANTS WITH ERYTHROBLASTOSIS FETALIS. III. P$sup 32$ UPTAKE AND INCORPORATION
The uptake and incorporation of P/sup 32/O/sub 4/ by the cord blood erythrocytes of normal and erythroblastotic infants and the erythrocytes of adults was studied by incubating specimens in ACD at 37 deg C in air. The uptake of P/sup 32/ from the plasma was slower in the infants than in the adults. It was possible to demonstrate an initial fast phase followed by a slow phase in all the plasma P/sup 32/ uptake experiments. The halfvalue time and the percentage turnover per minute indicated greater activity in the adults compared to the infants. The balance of movement of phosphorus was from red cells to plasma in all the experiments. No striking differences in phosphate metabolism were demonstrated between the normal and erythroblastotic newborns, except for the large turnover in mu of P per minute by the red cells of the latter during the first 20 minutes of incubation. Labeling of intraerythrocytic inorganic phosphorus (P/sub i/), adenosine triphosphate (ATP) and 2,3-diphosphoglycerate (2,3-DPG) proceeded more slowly in the infants than in the adults. The labeling of the erythrocyte 2,3-DPG of all the infants lagged far behind P and ATP whereas in the adults the relative specific activity (rsa) of 2,3-DPG had equalled or exceeded the rsa of P/sub i/ and ATP in four hours. It is suggested that there is decreased synthesis of phosphoglycer ates by the erythrocytes of the newborn which may be due to a lower level of glyceraldehyde-3-phosphate dehydrogenase activity. (auth)
- Research Organization:
- Milwaukee Blood Center
- NSA Number:
- NSA-16-012864
- OSTI ID:
- 4803549
- Journal Information:
- Blood (U.S.), Vol. Vol: 19; Other Information: Orig. Receipt Date: 31-DEC-62
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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