RIBONUCLEIC ACID METABOLISM DURING GROWTH OF EXCISED ROOT TIPS FROM NORMAL AND X-IRRADIATED CORN SEEDS

Journal Article · Sun Apr 01 04:00:00 EST 1962 · Biochim. et Biophys Acta

DOI:https://doi.org/10.1016/0006-3002(62)90981-2· OSTI ID:4789660

Cherry, J H

Growth of excised corn root tips was found to involve a degradation and loss of protein and ribonucleic acid. During growth (mostly cell elongation) of the normal excised root tips there is a major redistribution of ribonucleic acid among the subcellular components. The microsomal and soluble ribonucleic acids rapidly decline with a concomitant increase in the nuclear and mitochondrial fractions. There was little redistribution of ribonucleic acid in the root tips from x-irradiated seeds, since the transformation had already occurred as a result of x irradiation prior to incubation of the root tips. Incorporation of uracil into the subcellular ribonucleic acid fractions by excised root tips revealed that the greatest incorporation was in the nuclei, followed in order by mitochondria, microsomes, and the soluble fraction. X irradiation greatly enhanced the incorporation of uracil into ribonucleic acid. Growth of root ttps from normal and x irradiated seeds increased the ribonuclease activity as much as 2 to 4-fold. Root tips grown from x-irradiated seed had an initial ribonuclease activity 2 to 3-fold greater than normal tissue. (auth)

• Research Organization: Univ. of Illinois, Urbana
• NSA Number: NSA-16-017452
• OSTI ID: 4789660
• Journal Information: Biochim. et Biophys Acta, Journal Name: Biochim. et Biophys Acta Vol. Vol: 55
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

BIOLOGY AND MEDICINE
CEREALS
CYTOLOGY
DRUGS
ENZYMES
METABOLISM
PROTEINS
QUANTITY RATIO
RADIATION EFFECTS
RIBONUCLEIC ACID
ROOTS
SEEDS
SOLUBILITY
URACILS
X RADIATION