Effect of X-Irradiation on Nucleic Acids in Zea mays: II. On the Level of Ribonuclease Activity in Growing Seedlings
X irradiation of dry Zea mays seeds greatly enhanced the activity of RNase in growing seedlings, especially in the meristematic tissue (root tip and mesocotyl). Meristematic cells normally have a low level of RNase activity, but the activity increases with cell growth and maturation. The RNase activity of root tips grown from x-irradiated seeds was enhanced as much as fivefold. The distribution of RNase among the subcellular components of root tissue was not affected by x irradiation. X irradiation reduced the RNase activity in the scutellum, a storage tissue, which was consistent with the reduced uttlization of scutellar RNA. Studies on the incorporation of P/sup 32/ into the nucleotides by in vitro degradation of endogenous root tip RNA showed that incorporation was primarily into the pyrimidine nucleoside di- and triphosphates. EDTA stimulated RNase activity in root tip and mesocotyl homogenates but had little effect on scutellum homogenates.
- Research Organization:
- Univ. of Illinois, Urbana
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-17-001323
- OSTI ID:
- 4766584
- Journal Information:
- Radiation Research, Journal Name: Radiation Research Journal Issue: 5 Vol. 17; ISSN 0033-7587
- Publisher:
- Radiation Research Society
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
Similar Records
Effect of X-Irradiation on Nucleic Acids in Zea mays: I. Nucleic Acid Distribution and Metabolism in the Subcellular Components of Corn Seedlings Grown from Normal and X-Irradiated Seeds
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Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
ACIDITY
AGE
CEREALS
CONCENTRATION
DISTRIBUTION
EDTA
ENZYMES
HOMOGENEOUS REACTORS
ION EXCHANGE
LEACHING
MAGNESIUM OXIDES
METABOLISM
NITRATES
NUCLEIC ACIDS
ORE PROCESSING
PHOSPHATES
PHOSPHORUS 32
PLANT CELLS
PRECIPITATION
QUANTITY RATIO
RADIATION EFFECTS
RECOVERY
RIBONUCLEIC ACID
RNA-ASE
SEEDS
SEPARATION PROCESSES
SLURRIES
SOLUTIONS
SULFURIC ACID
TRACER TECHNIQUES
U3O8
URANIUM ORES
X RADIATION
ZEA MAYS
ACIDITY
AGE
CEREALS
CONCENTRATION
DISTRIBUTION
EDTA
ENZYMES
HOMOGENEOUS REACTORS
ION EXCHANGE
LEACHING
MAGNESIUM OXIDES
METABOLISM
NITRATES
NUCLEIC ACIDS
ORE PROCESSING
PHOSPHATES
PHOSPHORUS 32
PLANT CELLS
PRECIPITATION
QUANTITY RATIO
RADIATION EFFECTS
RECOVERY
RIBONUCLEIC ACID
RNA-ASE
SEEDS
SEPARATION PROCESSES
SLURRIES
SOLUTIONS
SULFURIC ACID
TRACER TECHNIQUES
U3O8
URANIUM ORES
X RADIATION
ZEA MAYS