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THE INDUCTION OF MUTATIONS IN SPERMATOCYTES OF DROSOPHILA MELANOGASTER WITH X-RAYS

Journal Article · · Intern. J. Radiation Biol.
OSTI ID:4784677
F/sub 1/ males of Drosophila melanogaster were irradiated with 1,000 rads x rays and then mated at the rate of two females per male per day for eight days following the irradiation. The frequencies of dominant lethals, translocations, sex-linked lethals, deleted X's and induced crossingover in the male were estimated in matings on days 5, 6, 7, and 8 following treatment. It is claimed that the sperm used in matings over these four sampling days was in the spermatocyte or early spermatid stage at the time of irradiation. Thus the frequencies of mutations recorded will give an estimate of the relative sensitivities of these various stages of germ cell development to x rays. Results show that sex-linked lethals and translocations follow similar patterns, reaching a peak on day 5, the level then droping off through days 6 and 7 to a low value on day 8. Dominant lethals show an increase from day 5 to a high level on day 6 which is maintained over days 7 and 8. Deleted X's and induced crossing- over increase from day 5 through days 6 and 7 to reach a peak on day 8. The relationship between the type of aberration studied and the sensitivity pattern of the treated germ-cells is discussed. (auth)
Research Organization:
Christid Hospital; and Holt Radium Inst., Manchester, Eng.
NSA Number:
NSA-17-000074
OSTI ID:
4784677
Journal Information:
Intern. J. Radiation Biol., Journal Name: Intern. J. Radiation Biol. Vol. Vol: 5
Country of Publication:
Country unknown/Code not available
Language:
English