Intratumoral distribution of tritiated fluorodeoxyglucose in breast carcinoma: I. Are inflammatory cells important?

Journal Article · Sun Oct 01 04:00:00 EDT 1995 · Journal of Nuclear Medicine

OSTI ID:478318

Brown, R S; Leung, J Y; Fisher, S J ^[1]

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1. Univ. of Michigan Medical Center, Ann Arbor, MI (United States)

To investigate the contribution of various tumor components to tumor [{sup 3}H]FDG uptake, the size of proliferative cell and macrophage populations and the extent of necrosis, inflammatory infiltration and granulation tissue formation were evaluated in syngeneic rat mammary cancers (RMC) grown in immunocompetent rats, an animal tumor model that closely mimics human breast carcinoma. Tissue components of breast cancers grown in female Lewis rats (n=6) were identified histologically and immunohistochemically. Tracer uptake was studied by quantitative autoradiography 2 hr after an intravenous injection of 100 {mu}Ci [{sup 3}H]FDG. RMC tumors were glandular, with small foci of necrosis and were surrounded by a thin layer of granulation tissue. Tumors retained approximately 4% of the injected FDG dose (1.9 {plus_minus} 0.27 {mu}Ci/g). Macrophages numbered 0.5% of total cancer cells (1.2 {plus_minus} 1.0 of 246 {plus_minus} 77) and 18.0% {plus_minus} 3.9% of the nuclei of cancer cells were proliferating cell nuclear antigen (PCNA) positive (52 {plus_minus} 27 of 293 {plus_minus} 55). FDG uptake (in apparent disintegrations per minute per microgram of protein) in the cancer cell was 47.3 {plus_minus} 5.6, with the highest uptake in foci of high tumor cell density (82.1 {plus_minus} 6.3). Lower levels of FDG uptake were found in necrotic areas (19.8 {plus_minus} 22.9), granulation tissue (26.9 {plus_minus} 9.2) and areas of inflammatory infiltraton (20.5 {plus_minus} 15.5). These data suggest that FDG-PET imaging of untreated breast cancer mainly reflects tracer uptake in cancer cells. 45 refs., 4 figs., 2 tabs.

• OSTI ID: 478318
• Journal Information: Journal of Nuclear Medicine, Journal Name: Journal of Nuclear Medicine Journal Issue: 10 Vol. 36; ISSN 0161-5505; ISSN JNMEAQ
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
56 BIOLOGY AND MEDICINE
APPLIED STUDIES
AUTORADIOGRAPHY
BIOLOGICAL MODELS
CARCINOMAS
CELL PROLIFERATION
FLUORODEOXYGLUCOSE
HISTOLOGICAL TECHNIQUES
MACROPHAGES
MAMMARY GLANDS
NECROSIS
RADIONUCLIDE KINETICS
RATS
TISSUE DISTRIBUTION
TRACER TECHNIQUES
TUMOR CELLS
UPTAKE