In vitro study of PET tumor tracers at normal and elevated media glucose levels

Journal Article · Wed May 01 04:00:00 EDT 1996 · Journal of Nuclear Medicine

OSTI ID:447761

Torizuka, T; Clavo, A C; Wahl, R L ^[1]

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1. Univ. of Michigan, Ann Arbor, MI (United States)

FDG uptake in tumors is decreased by hyperglycemia. Little is known about the effect of hyperglycemia on non-FDG PET tracer uptake in tumors. This study was designed to determine if PET tumor tracers are affected by chronic exposure of tumor cells to high media glucose levels. Human ovarian adenocarcinoma (HTB77IP3) cells normally grown at 100 mg/dl of glucose were grown in media with 100 or 300 mg/dl of glucose. At 20, 26 and 38 days after initial culture (6-7 days after subculture), uptakes of 3H-labeled FDG, Thymidine (Thy), Methionine (Met) and Leucine (Leu) into the cells (n=4) were determined at the same glucose level as growth media. Tracer uptake per 1 million cells was measured after a 60 min uptake period. Presented are percentage of tracer uptake of cells grown at 300 mg/dl of glucose relative to uptake of cells grown at 100 mg/dl of glucose (mean {plus_minus} SD of 20, 26, and 38 days culture). Paired t-tests were used to compare tracer uptake of cells grown and assayed at both glucose levels. P values <0.05 were considered significant. FDG uptake of cells grown and assayed at 300 mg/dl of glucose was significantly decreased, compared with uptake of cells grown and assayed at 100 mg/dl of glucose. By contrast, uptake of Thy, Met and Leu were not different between cells grown and assayed at 100 or 300 mg/dl of glucose. These results indicate that tumor uptake of Thy, Met and Leu do not depend on media glucose level and suggest that these tracers labeled with C-11 are suitable for hyperglycemic patients, in whom tumor FDG uptake is expected to be impaired.

• OSTI ID: 447761
• Report Number(s): CONF-960659--
• Journal Information: Journal of Nuclear Medicine, Journal Name: Journal of Nuclear Medicine Journal Issue: Suppl.5 Vol. 37; ISSN JNMEAQ; ISSN 0161-5505
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
CARBON 11
CARCINOMAS
CHRONIC EXPOSURE
FLUORODEOXYGLUCOSE
GLUCOSE
HYPERGLYCEMIA
LEUCINE
METHIONINE
PATIENTS
POSITRON COMPUTED TOMOGRAPHY
THYMIDINE
TRACER TECHNIQUES
TUMOR CELLS
UPTAKE