MUTATIONAL RESPONSE OF HABROBRACON OOCYTES IN METAPHASE AND PROPHASE TO ETHYL METHANESULFONATE AND NITROGEN MUSTARD

Journal Article · Sun Jul 01 04:00:00 EDT 1962 · Genetics (U.S.)

OSTI ID:4777173

Loebbecke, E A; von Borstel, R C

Ethyl methanesulfonate (EMS) and nitrogen mustard were used to induce dominant and recessive lethal mutations in the oocytes of the parasitic wasp Habrobracon. Oocytes in the first meiotic metaphase were found to be much more sensitive to EMS and nitrogen mustard than oocytes in first meiotic prophase when dominant lethality was the criterion. In this respect, the action of the chemical mutagens resembles that of x radiation. With recessive lethality as criterion, metaphase I and prophase I oocytes respond differently to x radiation and chemical mutagens. A 20-fold difference prevails after x radiation, with metaphase I being the more sensitive stage. With nitrogen mustard, metaphase I is three to five times more sensitive than prophase I, and with EMS, no significant difference was found between metaphase I and prophase I. (auth)

• Research Organization: Oak Ridge National Lab., Tenn.
• NSA Number: NSA-16-031406
• OSTI ID: 4777173
• Journal Information: Genetics (U.S.), Journal Name: Genetics (U.S.) Vol. Vol: 47; ISSN GENTA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ANIMAL CELLS
BIOLOGY AND MEDICINE
ETHYL RADICALS
GONADS
HABROBRACON
INSECTS
LIGHT
MEIOSIS
METABOLISM
METHANE
MITOSIS
MUTATIONS
NITROGEN COMPOUNDS
NITROGEN MUSTARD
ORGANIC SULFUR COMPOUNDS
PHOSPHATES
PHOSPHORUS 32
PHOTOCHEMISTRY
PLANTS
RADIATION EFFECTS
SEEDS
SULFONIC ACIDS
TRACER TECHNIQUES
ULTRAVIOLET RADIATION
X RADIATION