The Use of Gamma Radiation for the Preparation of Virus Vaccines

Journal Article · Wed Aug 01 04:00:00 EDT 1962 · Canadian Journal of Microbiology

DOI:https://doi.org/10.1139/m62-059· OSTI ID:4776554

Polley, John R.

Suspensions of the viruses (mumps, influenza A (PR8), A/sub 1/ (FM/sub 1/ ), B, and swine influenza A) were irradiated in a Co/sup 60/ cell with a dose of 1.5 x 10/sup 6/ rad, which is about 50% higher thin the dose calculated to be required for inactivation. A protective agent such as histidine or Na p- aminohippurite was added to purified suspensions of influenzi and mumps viruses. It was then possible to inactivate them while retaining most of the hemagglutination titer. It was demonstrated in mice that a vaccine prepared from a mouse-adapted virus (Shope's swine influenza) conferred protection against challenge by the live virus and produced an antibody response as measured by the hemagglutinationinhibition technique. Vaccines prepared with the viruses of influenza A(PR8), influenza B, and mumps were shown to produce antibody responses in guinea pigs as measured by the hemigglutination-inhibition and serum neutralization techniques. The use of gamma irradiation has an advantage over most chemical procedures because its dosage of inactivation can be more accurately controlled. (H.H.D.)

• Research Organization: Dept. of Health and Welfare, Ottawa
• Sponsoring Organization: USDOE
• NSA Number: NSA-17-001277
• OSTI ID: 4776554
• Journal Information: Canadian Journal of Microbiology, Journal Name: Canadian Journal of Microbiology Journal Issue: 4 Vol. 8; ISSN 0008-4166
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

AMINO ACIDS
ANTIBODIES
BIOLOGY AND MEDICINE
CHEMICAL REACTIONS
COBALT 60
COLOR
GAMMA RADIATION
GUINEA PIGS
HIPPURIC ACID
HISTIDINE
IMMUNITY
INFECTIONS
INFLUENZA
IRRADIATION
LETHAL DOSE
MASS
MEASURED VALUES
MEDICINE
MICE
MOLECULES
OXIDATION
PREPARATION
PUMPS
QUANTITY RATIO
RADIATION CHEMISTRY
RADIATION DOSES
RADIATION EFFECTS
RADIATION PROTECTION
SUSPENSIONS
USES
VACCINES
VIRUSES
X RADIATION