THE EFFECT OF $gamma$-RADIATION ON THE SYNTHESIS OF DNA IN ISOLATED CELL NUCLEI OF THE THYMUS. II. A COMPARATIVE STUDY OF THE DNA SYNTHESIS IN THE THYMUS NUCLEI AFTER EXPOSURE OF ISOLATED NUCLEI TO $gamma$-RADIATION AND AFTER WHOLE-BODY IRRADIATION OF THE ANIMALS (in Russian)
It was previously established that the irradiation of the tissue impedes the DNA synthesis; in this connection it was previously shown (Doklady Akad. Nauk SSSR 91: 1183 (1953)) that incorporation of P/sup 32/ into the nucleic acids of the spleen is greatly dependent on the fact whether that organ alone or the whole animal was irradiated. In order to clarify the direct effect of radiation, this problem was further studied by introducing labeled predecessors into the DNA molecule, using tissue nuclei separated from the cytoplasm. The formation of purine and pyrimidine bases was investigated by using C/suip 14/--formiate and C/ sup 14/- adenine. The first conmpound is incorporated only in the thymine and the second only in the DNA adenine; this may be used in comparing the effect of ionizing radiations on these reactions. Results indicated that after total irradiation of rats to 1000 r, the ability of the tissue nuclei isolated from the thymus to incorporate either of these compounds is greatly reduced. Irradiation of separate tissue nuclei of the thymus resulted in a slight depression of the incorporation process of the C/sup 14/--formiate into the DNA thymine while the corresponding adenine process was hardly affected. In general, irradiation in vivo caused more injury than exposure of separate organs to ionizing radiation. (TTT)
- Research Organization:
- Inst. of Biological Physics, Academy of Sciences, USSR
- NSA Number:
- NSA-16-030255
- OSTI ID:
- 4770790
- Journal Information:
- Radiobiologiya (U.S.S.R.) For English translation see Radiobiology (Engl. Transl.), Vol. Vol: 2; Other Information: Orig. Receipt Date: 31-DEC-62
- Country of Publication:
- Country unknown/Code not available
- Language:
- Russian
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