A 5.5-Mb high-resolution integrated map of distal 11q13

Journal Article · Sat Feb 01 04:00:00 EST 1997 · Genomics

DOI:https://doi.org/10.1006/geno.1996.4460· OSTI ID:476885

Merscher, S ^[1]; Bekri, S; De Leeuw, B ^[2]

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1. Universite de Nice, Sophia (France)
2. Universite de Nice, Sophia (France); and others

The distal part of 11q13, which contains several genes relevant to human diseases, has been poorly mapped as part of genome-wide mapping efforts. In the prospect of drawing a fine-scale integrated map of the area containing KRN1 and OMP, we have established a framework of markers by hybridization to DNA of somatic cell hybrids and by fluorescence in situ hybridization (FISH) on metaphase chromosomes. The probes studied were used to isolate 27 YACs and 16 cosmids that could be organized in three contigs covering approximately 6 Mb. These contigs were separated by two gaps that are likely to contain sequences underrepresented in YAC libraries. They were then integrated based on long-range restriction mapping and DNA-fiber FISH into a high-resolution physical map, which covers a 5.5-Mb region and includes 36 anonymous markers and 10 genes. This map will be used to search for genes within the 2/3 of this region where none have been localized as yet. It will also lay the ground for the characterization of an amplicon surrounding GARP in breast cancer and for the search of disease genes within this region. 42 refs., 2 figs., 2 tabs.

• OSTI ID: 476885
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 3 Vol. 39; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
BIOLOGICAL MARKERS
CHROMOSOMAL ABERRATIONS
CONTIGS
COSMIDS
DESIGN
DNA SEQUENCING
DNA-CLONING
ELECTROPHORESIS
FLUORESCENCE
GENE AMPLIFICATION
GENES
GENETIC MAPPING
HEREDITARY DISEASES
HUMAN CHROMOSOMES
HYBRIDIZATION
IN-SITU HYBRIDIZATION
MAMMARY GLANDS
NEOPLASMS
ONCOGENIC TRANSFORMATIONS
PLASMIDS
POLYMERASE CHAIN REACTION
PROBES
RESOLUTION
SOMATIC CELLS