Physical mapping of human chromosome segment 9q34
- Harvard Medical School, Boston, MA (United States); and others
A high resolution physical map of the {approximately}15 Mb that constitute human chromosome region 9q34 is being constructed. YACs have been identified with 138 9q34 markers including 40 genetic markers (STS, SSR), 8 known genes and 90 exons isolated from cosmids localized to 9q34 by FISH or localized to 9q34 by hybridization to somatic cell hybrid mapping panels. In addition, Alu-PCR products (62) and end clones (29) from 9q34 YACs have been utilized to identify additional overlapping clones in the region. A total of 229 markers have identified 257 YACs (62 unique and 195 redundant) that have been organized into 41 contigs and cover > 12 Mb of this region. Fifteen of these contigs are sub-localized within 9q34 based on the presence of a genetic marker, and 26 contigs are localized only to 9q34 based on the origin of the marker they contain. The location of these contigs in 9q34 is being independently confirmed by FISH of YAC Alu-PCR products. Contig extension is being pursued through hybridization of individual YAC Alu-PCR products to Alu-PCR products of clones in a chromosome 9 YAC library and by chromosome walking from YAC end clones. This has resulted in the extension of 14 contigs and the reduction of 5 smaller contigs to 2 larger contigs. In addition, a 1.7 Mb complete, overlapping contig has been constructed in the TSC1 candidate region. It is anticipated that a cloned physical map of 9q34 will aid in the identification of this and other disease genes localized to this chromosome segment.
- OSTI ID:
- 134396
- Report Number(s):
- CONF-941009--
- Journal Information:
- American Journal of Human Genetics, Journal Name: American Journal of Human Genetics Journal Issue: Suppl.3 Vol. 55; ISSN AJHGAG; ISSN 0002-9297
- Country of Publication:
- United States
- Language:
- English
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