Mapping of the mouse actin capping protein {alpha} subunit genes and pseudogenes

Journal Article · Sat Feb 01 04:00:00 EST 1997 · Genomics

DOI:https://doi.org/10.1006/geno.1996.4506· OSTI ID:476877

Hart, M C; Korshunova, Y O; Cooper, J A ^[1]

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1. Washington Univ. School of Medicine, St. Louis, MO (United States)

Capping protein (CP), a heterodimer of {alpha} and {beta} subunits, is found in all eukaryotes. CP binds to the barbed ends of actin filaments in vitro and controls actin assembly and cell motility in vivo. Vertebrates have three {alpha} isoforms ({alpha}1, {alpha}2, {alpha}3) produced from different genes, whereas lower organisms have only one gene and one isoform. We isolated genomic clones corresponding to the a subunits of mouse CP and found three {alpha}1 genes, two of which are pseudogenes, and a single gene for both {alpha}2 and {alpha}3. Their chromosomal locations were identified by interspecies backcross mapping. The {alpha}1 gene (Cappa1) mapped to Chromosome 3 between D3Mit11 and D3Mit13. The {alpha}1 pseudogenes (Cappa1-ps1 and Cappa1-ps2) mapped to Chromosomes 1 and 9, respectively. The {alpha}2 gene (Cappa2) mapped to Chromosome 6 near Ptn. The {alpha}3 gene (Cappa3) also mapped to Chromosome 6, approximately 68 cM distal from Cappa2 near Kras2. One mouse mutation, de, maps in the vicinity of the {alpha}1 gene. No known mouse mutations map to regions near the {alpha}2 or {alpha}3 genes. 29 refs., 3 figs., 1 tab.

• OSTI ID: 476877
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 3 Vol. 39; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
ACTIN
BIOLOGICAL MARKERS
CHROMOSOMES
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
GENE MUTATIONS
GENETIC MAPPING
HYBRIDIZATION
MICE
POLYMERASE CHAIN REACTION
PROTEINS
RFLPS
STRUCTURE-ACTIVITY RELATIONSHIPS