TISSUE TRANSPLANTATION: HOMOGRAFT ANTIGENS, IONIZING RADIATION AND OTHER FACTORS INFLUENCING HOMOGRAFT SURVIVAL. Progress Report, June 1, 1961-May 31, 1962

Technical Report · Thu Oct 31 04:00:00 EST 1963

OSTI ID:4768571

Dammin, G J; Corson, J M; Mann, Jr, L T

Progress is reported in studies of reactions to transplanted organs and tissues in man and animals. Modifications were made in the method for extracting antigens from mouse spleen cells. The essential constituents of this active fraction are protein and lipid. In studies of the canine splenic homograft, it was concluded that an early graft versus host reaction was present for a period probably not exceeding 48 hours. This was followed promptly by a progressive increase in cellularity of the splenic homograft. Suppression of the host's marrow and lymphoid tissues with nitrogen mustard prevented the lymphocytic and plasmocytic infiltration of the splenic homograft and prolonged its survival. Few dogs survived long enough to determine precisely how the host was modified, but it did appear that there might be a prolongation of the graft versus host response induced by the nitrogen mustard. It was plain that the aim of attaining a state of mutual tolerance had not been achieved. The best survival achieved thus far for the canine renal homograft has followed a program consisting of the initial administration of 6 mercaptopurine, with BWS7-322 given as a daily dose, with periodic addition of Actinomycin C. With this program, more than half of the dogs survived more than 30 days, with the renal homograft as the only renal tissue in the recipient. A similar program has been followed in man for the first time and has resulted in the longest survival obtained thus far with chemical treatment only. The characteristics of synthetic polypeptides as antigens were defined further. Amino acid content as well as sequence are important determinants of anitigenicity. This is borne out also by examination of the inhibitory effects on antigen-antibody combination by amino acids and dipeptides. (auth)

• Research Organization: Harvard Univ., Boston. Medical School
• NSA Number: NSA-17-005762
• OSTI ID: 4768571
• Report Number(s): AD-276039
• Country of Publication: United States
• Language: English

Related Subjects

AMINO ACIDS
ANIMAL CELLS
ANIMALS
ANTIBIOTICS
ANTIGENS
BIOLOGY AND MEDICINE
BONE MARROW
CHEMICALS
DOGS
HETEROCYCLICS
IMMUNITY
KIDNEYS
LIPIDS
LYMPH SYSTEM
MAN
MICE
NITROGEN COMPOUNDS
NITROGEN MUSTARD
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PROTEINS
PURINES
RADIATION EFFECTS
SPLEEN
TISSUES
TRANSPLANTS