A YAC contig of the human CC chemokine genes clustered on chromosome 17q11.2
- Kumamoto Univ. Medical School, Honjo (Japan)
- Kumamoto Univ. Medical School, Honjo (Japan); and others
CC chemokines are cytokines that attract and activate leukocytes. The human genes for the CC chemokines are clustered on chromosome 17. To elucidate the genomic organization of the CC chemokine genes, we constructed a YAC contig comprising 34 clones. The contig was shown to contain all 10 CC chemokine genes reported so far, except for one gene whose nucleotide sequence is not available. The contig also contains 4 CC chemokine-like genes, which were deposited in GenBank as ESTs and are here referred to as NCC-1, NCC-2, NCC-3, and NCC-4. Within the contig, the CC chemokine genes were localized in two regions. In addition, the CC chemokine genes were localized in two regions. In addition, the CC chemokine genes were more precisely mapped on chromosome 17q11.2 using a somatic cell hybrid cell DNA panel containing various portions of human chromosome 17. Interestingly, a reciprocal translocation t(Y;17) breakpoint, contained in the hybrid cell line Y1741, lay between the two chromosome 17 chemokine gene regions covered by our YAC contig. From these results, the order and the orientation of CC chemokine genes on chromosome 17 were determined as follows: centromere-neurofibromatosis 1-(MCP-3, MCP-1, NCC-1, I-309)-Y1741 breakpoint-RANTES-(LD78{gamma}, AT744.2, LD78{beta})-(NCC-3, NCC-2, AT744.1, LD78{alpha})-NCC-4-retinoic acid receptor {alpha}-telomere. 22 refs., 1 fig., 2 tabs.
- OSTI ID:
- 476783
- Journal Information:
- Genomics, Journal Name: Genomics Journal Issue: 2 Vol. 34; ISSN GNMCEP; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BASIC STUDIES
AMINO ACIDS
BIOLOGICAL MARKERS
CHROMOSOMAL ABERRATIONS
CONTIGS
DESIGN
DNA SEQUENCING
DNA-CLONING
GENES
GENETIC MAPPING
HUMAN CHROMOSOME 17
HUMAN Y CHROMOSOME
HYBRIDIZATION
LEUKOCYTES
NUCLEOTIDES
POLYMERASE CHAIN REACTION
SOMATIC CELLS
STRUCTURE-ACTIVITY RELATIONSHIPS