Assembly of a 1-Mb restriction-mapped cosmid contig spanning the candidate region for Finnish congenital nephrosis (NPHS1) in 19q13.1

Journal Article · Sat Jun 01 04:00:00 EDT 1996 · Genomics

DOI:https://doi.org/10.1006/geno.1996.0270· OSTI ID:476779

Olsen, A S; Georgescu, A; Johnson, S; Carrano, A V ^[1]

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1. Lawrence Livermore National Lab., CA (United States)

We describe the assembly of a 1-Mb cosmid contig and restriction map spanning the candidate region for Finnish congenital nephrosis (NPHS1) in 19q13.1. The map was constructed from 16 smaller contigs assembled by fingerprinting, a BAC and a PAC clone, and 42 previously unmapped cosmids. In most cases, single-step cosmid walks were sufficient to join two previously assembled contigs, and all but one gap was filled from this cosmid contig library. The remaining gap of about 19 kb was spanned with a single BAC and a single PAC clone. EcoRI mapping of a dense set of overlapping clones validated the assembly of the map and indicated a length of 1040 kb for the contig. This high-resolution clone map provides an ideal resource for gene identification through cDNA selection, exon trapping, and DNA sequencing. 10 refs., 1 fig.

• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 476779
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 2 Vol. 34; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
BIOLOGICAL MARKERS
CONTIGS
COSMIDS
DESIGN
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
EXONS
FINLAND
FLUORESCENCE
GENES
GENETIC MAPPING
HEREDITARY DISEASES
HUMAN CHROMOSOME 19
HUMAN POPULATIONS
RECESSIVE MUTATIONS
RESOLUTION
UROGENITAL SYSTEM DISEASES