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Niche adjustment for bioaugmentation with Pseudomonas sp. strain KC

Conference ·
OSTI ID:474239
; ;  [1];  [2];  [3]
  1. Michigan State Univ., East Lansing, MI (United States)
  2. Michigan State Univ., Noblesville, IN (United States)
  3. Golder Associates, Inc., East Lansing, MI (United States)
To be effective, novel organisms introduced into the environment must be able to survive and compete with indigenous organisms. However, to minimize the possibility of ecological disturbance, colonization should ideally be constrained. A possible solution is to create a temporary niche for the introduced organism. Alkalinity addition creates just such a niche by reducing the bioavailability of essential trace metals, such as iron, thereby favoring organisms with efficient trace metal scavenging systems. The authors evaluated alkaline pH adjustment with Pseudomonas sp. strain KC, a denitrifying aquifer organism that degrades carbon tetrachloride (CT) under denitrifying conditions. They compared the kinetic parameters of strain KC with those of its potential competitors (other denitrifiers) in groundwater from a CT-contaminated aquifer at Schoolcraft, Michigan. Under moderately alkaline conditions, strain KC had a higher maximum specific growth rate and yield. With alkaline adjustment, strain KC grew and degraded CT in columns containing aquifer solids from the Schoolcraft site and in slurries of aquifer material from Hanford, Washington. Upon reducing pH, a rapid decline in the KC population followed.
Sponsoring Organization:
Michigan State Government, Lansing, MI (United States); Environmental Protection Agency, Washington, DC (United States)
OSTI ID:
474239
Report Number(s):
CONF-950483--; ISBN 1-57477-004-7
Country of Publication:
United States
Language:
English

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