Monitoring and control of pseudomonas sp. strain KC for in-situ bioaugmentation of carbon tetrachloride contaminated sites
Conference
·
OSTI ID:111533
- Michigan State Univ., East Lansing, MI (United States); and others
Pseudomonas sp. strain KC converts carbon tetrachloride (CT) to carbon dioxide, formate, and non-volatile product(s) without the production of chloroform under denitrifying conditions. Most other organisms that transform CT under such conditions do so slowly and/or produce chloroform, making strain KC an attractive candidate for bioaugmentation applications. For strain KC, the mechanism of CT transformation involves secretion of a low molecular weight factor under iron-limiting conditions. The secreted factor fortuitously transforms CT and is activated for transformation by live whole cells. To determine whether the capabilities of strain KC can be applied for in-situ remediation of CT contamination, the Michigan Department of Natural Resources is supporting a field experiment in a CT-contaminated aquifer at Schoolcraft, MI. The present report describes laboratory work funded by the Great Lakes and Mid-Atlantic Hazardous Substance Research Center in support of the field project. These laboratory efforts have focused on: (1) isolation and identification of the secreted factor, (2) development and testing of a laboratory-scale model aquifer and numerical simulator to describe establishment and maintenance of a CT-transforming zone, (3) development and testing of a gene probe for detection and monitoring of strain KC, (4) identification of characteristic biochemical markers for strain KC, and (5) identification of key transport and retention mechanisms for strain KC.
- OSTI ID:
- 111533
- Report Number(s):
- CONF-9504110--
- Country of Publication:
- United States
- Language:
- English
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