PROPHYLACTIC VACCINATION WITH VACCINES OF BACTERIA INACTIVATED BY X IRRADIATION

Journal Article · Mon Jan 01 04:00:00 EST 1962 · Zentr. Bakteriol., Parasitenk., Abt. I. Orig.

OSTI ID:4739856

Beutel, H

Mouse protection tests were conducted with vaccines prepared from Brucella abortus or Listeria monocytogenes by means of x rays or formaldehyde. B. abortus (Buck 19 strain) was killed by irradiation with 50-kv x rays while suspended in saline solution. The absolute sterilizing dose for 10/sup 10/ bacteria was 1.5 Mr. Vaccine prepared in this way, or by treatment with HCHO at a concentration of 0.2%, was injected with Al(OH)/sub 3/ in mice. When the mice were subsequently challenged with living bacteria, the survival rate was 43% in mice immunized with radioinactivated vaccine, 30% in those given formalized vaccine, and 0% in unvaccinated controls. Vaccines were similarly prepared and tested using L. monocytogenes, except the x-ray sterilizing dose was 1.0 Mr. With both irradiated and formalized vaccines the survival rate of challenged mice was 5%, compared with 0% in unvaccinated controls. Although the results indicate that there is little difference between the protection afforded by vaccines prepared with x rays or re HCHO, the irradiated vaccines were somewhat better tolerated by the mice than formalized vaccines. (H.H.D.)

• Research Organization: Universitat, Munich
• NSA Number: NSA-17-017701
• OSTI ID: 4739856
• Journal Information: Zentr. Bakteriol., Parasitenk., Abt. I. Orig., Journal Name: Zentr. Bakteriol., Parasitenk., Abt. I. Orig. Vol. Vol: 187
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ALDEHYDES
ALUMINUM HYDROXIDES
ANTIGENS
BACTERIA
BIOLOGY AND MEDICINE
FORMIC ACID
IMMUNITY
IRRADIATION
MICE
PREPARATION
RADIATION DOSES
SALTS
SOLUTIONS
VACCINES
X RADIATION