Studies on the Incorporation of Tritiated Thymidine in Desoxyribonucleic Acid in Mouse Tissues and on Its Radiation Effects
Journal Article
·
· International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine
Studies are reported which were designed to check for possible disadvantageous effects of labeling DNA with tritiated thymidine of high specific activity in long-term experiments. Three series of mice were injected with 200 c, 20 c, and 5 c of the substance per mouse, and one series received 4 c per mouse of C¹⁴-labeled thymidine. The total activity was recorded, and the degree of labeling was investigated in autoradiographs at intervals up to 120 days after injection in seven different tissues. The results indicate that cellular death due to radiation from the label occurs in mice labeled with a dose of 200 c, and possibly also with a dose of 20 c per mouse (about 1 c/ g). The incorporation of thymidine in several tissues does not increase proportionately to the amount available, indicating a pool effect that might be due to thymidine existing as a normal intermediate. The findings may have implications for the use of tritiated thymidine as a label for DNA in autoradiographic studies. (auth)
- Research Organization:
- Institutt for Atomenergi, Kjeller pr. Lillestrom, Norway
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-17-019847
- OSTI ID:
- 4733521
- Journal Information:
- International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine, Journal Name: International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine Journal Issue: 2 Vol. 6; ISSN 0020-7616
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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