THE IN VIVO INCORPORATION OF TRITIATED CYTIDINE AND TRITIATED THYMIDINE BY THE CESTODE, HYMENOLEPIS MICROSTOMA
The mouse bile duct cestode, Hymenolepis microstoma, was exposed in vivo in separate experiments to tiitiated cytidine and tritiated thymidine, respectively. Nucleolar and nuclear labeling, by cytidine, and nuclear labeling, by thymidine, as revealed by autoradiographic methods, occurred in both host and cestode tissues. As expected, only mitotically or synthetically active nuclei were labeled, those of the neck, developing organs, and embryos of the cestode. Bile-duct epithelium and pancreatic acinar cells, autoradiographed as a control, showed the same degree and localization of cytidine labeling as the active cells of the cestode. The assimilation of cytidine amd thymidine by direct absorption from the fluid contents of the bile duct implies that cestodes can absorb such materials from their immediate environment. (auth)
- Research Organization:
- Tennessee. Univ., Knoxville
- NSA Number:
- NSA-17-040490
- OSTI ID:
- 4647310
- Report Number(s):
- TID-18025
- Country of Publication:
- United States
- Language:
- English
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ANIMAL CELLS
ANIMALS
BILE DUCTS
BIOLOGY AND MEDICINE
CELL NUCLEI
CESTODES
CYTIDINE
DIFFUSION
EMBRYOS
GLANDS
LABELLED COMPOUNDS
LIVER
METABOLISM
MICE
MITOSIS
NUCLEIC ACIDS
ORGANIC NITROGEN COMPOUNDS
PANCREAS
PARASITES
RADIOAUTOGRAPHY
REPRODUCTION
THYMIDINE
TISSUES
TITANIUM
TRACER TECHNIQUES