Incorporation of inorganic P³² into phospholipids of brain slices. Effect of certain tranquilizing drugs

Journal Article · Tue Jan 01 04:00:00 EST 1963 · Biochemistry and Cell Biology

DOI:https://doi.org/10.1139/o63-131· OSTI ID:4725985

Magee, W. L.; Rossiter, R. J.

Promazine, promethazine, tetrameprazine and WY 1172 resembled chlorpromazine in that when they were added in a concentration of 0.1 mM to slices of guinea pig brain respiring in a suitable medium they stimulated the incorporation of inorganic P³² into the phospholipids of the slices. With one of the drugs, promethazine, the concentration of 0.1 mM was found to cause no significant increase in respiration, in aerobic glycolysis, or in the concentration of phosphocreatine. In higher concentrations (1.0 mM), all of the compounds inhibited the labeling of phospholipid. Promethazine caused a reduction in respiration and in the concentration of phosphocreatine, accompanied by an increase in aerobic glycolysis. Methylene blue did not stimulate the labeling of phospholipid in brain slices. Azacyclonol, pipradrol, and mepazine also stimulated phospholipid labeling in low concentrations and inhibited the labeling at higher concentrations. Piperidine and benzhydrol did not stimulate phospholipid labeling at the concentration that was most effective for azacyclonol. Low concentrations of benzhydrol, however, caused a slight stimulation. Meprobamate and phenaglycodol had either little or no effect. Most of the substances tested inhibited phospholipid labeling when they were added in sufficiently high concentrations.

• Research Organization: Univ. of Western Ontario, London
• Sponsoring Organization: USDOE
• NSA Number: NSA-17-023044
• OSTI ID: 4725985
• Journal Information: Biochemistry and Cell Biology, Journal Name: Biochemistry and Cell Biology Journal Issue: 5 Vol. 41; ISSN 0829-8211
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
AMINES
BRAIN
CHEMICAL REACTIONS
DRUGS
EFFICIENCY
ENZYMES
GUINEA PIGS
HETEROCYCLICS
LABELLED COMPOUNDS
LIPIDS
METHYL RADICALS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXIDATION
PHENOTHIAZINES
PHOSPHORUS 32
PROMAZINE
PROPYL RADICALS
QUANTITY RATIO
REDUCTION
TESTING