Determination of Fe ⁵⁵ and Fe ⁵⁹ Mixtures in Blood by Liquid Scintillation Counting

Journal Article · Mon Jan 01 04:00:00 EST 1962 · Scandinavian Journal of Clinical and Laboratory Investigation

DOI:https://doi.org/10.3109/00365516209051264· OSTI ID:4714815

Jenner, H.; Öbrink, K. J.

Iron-55 and Fe⁵⁹ were determined simultaneously in blood samples after the organic material had been destroyed by nitric and perchloric acids. The Fe was precipitated as hydroxide, dissolved in oxalic acid and electroprecipitated on a platinum rod. It was then dissolved as perchlorate, the solution evaporated to a small volume and added to a solution of diphenyloxazole in toluene and absolute ethanol. The activity was determined in a single photomultiplier tube liquid scintillation counter by means of pulse height analysis. The accuracy of the method (standard deviation) was 3.4%. Slightly more than 1.3% of the error was the combined standard error due to countering statistics, and the remaining 2% due to chemical, technical, and instrumental errors. The possible influence of different amounts of total Fe in the sample was also examined, because quenching by Fe might have caused a spectrum shift. In samples containing 2.5 to 20.0 mg stable Fe in addition to identical amounts of Fe⁵⁵ and Fe⁵⁹, no evidence of such an effect on the results was detected.

• Research Organization: Uppsala Univ.
• Sponsoring Organization: USDOE
• NSA Number: NSA-17-021640
• OSTI ID: 4714815
• Journal Information: Scandinavian Journal of Clinical and Laboratory Investigation, Journal Name: Scandinavian Journal of Clinical and Laboratory Investigation Journal Issue: 5 Vol. 14; ISSN 0036-5513
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

BLOOD
CHEMISTRY
DECOMPOSITION
ELECTRODEPOSITION
ERRORS
ETHANOL
EVAPORATION
IRON
IRON 55
IRON 59
IRON HYDROXIDES
LIQUIDS
MIXING
NITRIC ACID
ORGANIC NITROGEN COMPOUNDS
OXALIC ACID
PERCHLORIC ACID
PHOTOMULTIPLIERS
PLATINUM
PRECIPITATION
PULSES
QUANTITATIVE ANALYSIS
QUANTITY RATIO
SCINTILLATION COUNTERS
SENSITIVITY
SOLUTIONS
SPECTRAL SHIFT
STANDARDS
TISSUES
TOLUENE