BIOCHEMICAL IMPLICATIONS OF PRO-OXIDANTS AND ANTIOXIDANTS
Lipid peroxides can be detected in intact adipose tissue cells but have not been shown to be present in other normal cells. On injury of such cells, they are rapidly formed. This post-injury formation is dependent on traces of inorganic iron liberated from a protein- or hematin-bound state. Ascorbic acid acts as a co-oxidant in the reaction. The iron-catalyzed reaction can be inhibited by the addition of chelating agents, including free fatty acids, or by antioxidants such as vitamin E added in vitro. Adding excess vitamin E to the diet also decreases lipid peroxidation in the injured cells. Tissues in which cell division is continuously occurring (bone marrow, tumors, intestinal mucosa) produce no lipid peroxides even after the cells are injured. Antioxidant activity in these cells must be exceptionaliy high. Analysis of the conditions in intestinal mucosa shows that phospholipase activity can be correlated with antioxidant activity. After irradiation, the virtual absence of a cofactor reduces the phospholipase activity and reduces the antioxidant to the same extent. The nature of the antioxidant in bone marrow and tumor is still unknown. (auth)
- Research Organization:
- Duke Univ., Durham, N.C.
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-17-024975
- OSTI ID:
- 4714346
- Journal Information:
- Radiation Res., Suppl. 3, Other Information: Orig. Receipt Date: 31-DEC-63
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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