PEROXIDE FORMATION IN PLANT CELL AFTER IONIZING RADIATION EFFECT
Biochemical changes were investigated in bean and barley seedlings after exposure to 1000 to 20,000 r x irradiation. Lipoxidase, an enzyme oxidizing unsaturated fatty acids, increased 30 to 50% within 24 hr after irradiation. Four hr after 20,000 r, the unsaturated fatty acid content of bean seedlings fell sharply, and lipid peroxide values rose markediy after 1000 to 10,000 r. Most of the lipid peroxide was present in the free lipid fraction of irradiated seedlings; very little bound lipid peroxide was present after irradiation. Since lipid peroxides have been shown to be cytotoxic and to inhibit mitosis in vitro, inhibit enzyme systems, and depolymerize DNA, part of the injurious effect of irradiation on the cell may be thus explained. Peroxidase activity could be destroyed by steaming the seedlings. When such steamed leaves were irradiated with 10,000 r, no lipid peroxides were formed, indicating their formation in unheated leaves results from the action of the enzyme. The changes in cellular lipids following irradiation may be related to postirradiation changes observed in cell membranes, mitochondria, microsomes, and nuclei. (H.H.D.)
- Research Organization:
- Inst. of Biochemistry, Moscow
- NSA Number:
- NSA-17-023129
- OSTI ID:
- 4712623
- Journal Information:
- Pathol. Biol., Semaine Hop., Journal Name: Pathol. Biol., Semaine Hop. Vol. Vol: 9
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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