AN APPROACH TO THE CHARACTERIZATION OF STEM CELLS IN MOUSE BONE MARROW

Conference · Sun Dec 02 04:00:00 EST 1962

OSTI ID:4706592

Cudkowiez, G.; Upton, A. C.; Smith, L. H.; Gosslee, D. G.; Hughes, W. L.

The competence of bone marrow to promote recovery of hemopoietic function in irradiated recipient mice was observed to decrease on serial transplantation. Evidence is presented that the protective ability of the serially transplanted marrow can be reconstituted by addition of lymphoid cells harvested from normal lymph nodes. The nature and cellular basis of variations in the proliferative capacity of a given marrow cell population in the course of syngeneic (isologous) transplantation were investigated. A quantitative measure of this capacity of the marrow was obtained by determining the ability of the marrow to form colonies in the spleens of irradiated recipient mice and by measuring incorporation of I/sup 131/-labeled iododeoxyuridine, a specific desoxyribonucleic acid precursor, in such spleens. The proliferative capacity, as evaluated by these techniques, was related to the cellular constitution of the marrow, as determined by differential cytologic analysis of marrow smears, in an attempt to identify the responsible stem cells. (auth)

• Research Organization: Oak Ridge National Lab., Tenn.; and Brookhaven National Lab., Upton, N.Y.
• Sponsoring Organization: US Atomic Energy Commission (AEC)
• DOE Contract Number: W-7405-ENG-26
• NSA Number: NSA-17-030286
• OSTI ID: 4706592
• Report Number(s): CONF-29-1
• Country of Publication: United States
• Language: English

Related Subjects

BIOLOGY AND MEDICINE
BLOOD FORMATION
BONE MARROW
CONFERENCE
CYTOLOGY
EFFICIENCY
HETEROCYCLICS
IODINE 131
IRRADIATION
LABELLED COMPOUNDS
LYMPH NODES
LYMPH SYSTEM
MICE
MITOSIS
NUCLEIC ACIDS
ORGANIC NITROGEN COMPOUNDS
RADIATION PROTECTION
RECOVERY
SPLEEN
TRANSPLANTS
URIDINE
VARIATIONS