HISTOCHEMICAL CHANGES IN THE LEUCOCYTES IN ACUTE RADIATION SICKNESS

Journal Article · Fri Jan 01 04:00:00 EST 1960 · Vop. Radiobiol., Akad. Nauk Arm. SSR, Sektor Radiobiol., Sb. Tr.

OSTI ID:4704520

Pareyshvili, E A

Early metabolic disturbances in the leucocytes after x-irradiation were carried out by histochemical methods on 100 white mice which were subjected to a lethal dose of 800 r. The ribonucleic acid content of the leucocytes as determined by the Brachet method began to fall 10 minutes after irradiation, and after 72 hours the cells had all lost their staining power, except for a very occasional cell which had presumably just been released from the bone marrow. In preparations stained by the Feulgen method to demonstrate deoxyribonnucleic acid, some loss of reticulation was noted in the nuclei of the leucoctyes 20 minutes after irradiation, and after 3 hours the staining had become diffuse and reduced in intensity. The decline in staining power continued until 72 hours, but deoxyribonucleic acid did not disappear completely and some was still present at death. The catalase activity of the neutrophils began to rise 5 minutes after irradiation and fell again after 3 hours. The fat metabolism of the cells was relatively radio-resistant; fat did not begin to accumulate in the cells until after 3 hours, and the changes were not marked until after 12 hours. (OTS)

• Research Organization: Radiobiological Section, Academy of Sciences, Armenian SSR
• NSA Number: NSA-17-030443
• OSTI ID: 4704520
• Journal Information: Vop. Radiobiol., Akad. Nauk Arm. SSR, Sektor Radiobiol., Sb. Tr., Journal Name: Vop. Radiobiol., Akad. Nauk Arm. SSR, Sektor Radiobiol., Sb. Tr. Vol. Vol: 1
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

BIOCHEMISTRY
BIOLOGY AND MEDICINE
BONE MARROW
CATALASE
CYTOLOGY
DNA
DYES
ENZYMES
LETHAL DOSE
LEUCOCYTES
LIPIDS
METABOLISM
MICE
NUCLEIC ACIDS
ORGANIC ACIDS
QUANTITY RATIO
RADIATION SICKNESS
RADIOSENSITIVITY
RIBONUCLEIC ACID
VARIATIONS
X RADIATION