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Mutagenic response to benzene and tris(2,3-dibromopropyl)-phosphate in the lambda lacI transgenic mouse mutation assay: A standardized approach to in vivo mutation analysis

Journal Article · · Environmental and Molecular Mutagenesis
; ;  [1];  [2]
  1. Stratagene and Recombinant Biocatalysis, Inc., La Jolla, CA (United States)
  2. SRI International, Menlo Park, CA (United States)

The genotoxic response of benzene and tris(2,3-dibromopropyl)-phosphate (TDBP) have been evaluated in several tissues using the standardized lambda/lacI (Big Blue{reg_sign}) transgenic mouse mutation assay. Separate groups of four to give male B6C3F, transgenic lambda/lacl mice were given oral administrations of benzene or TDBP at varying concentrations. Tissues evaluated include lung, bone marrow, and spleen in benzene-treated animals, and liver, kidney, and stomach in TDBP-treated animals. Significant increases in lacI mutations were observed in the spleen and bone marrow of benzene treated mice, and the kidneys of TDBP-treated mice. Where applicable, mutagenesis patterns of tissue sensitivity were consistent with what has been observed previously in other assays. In addition, mutagenicity in tissues not traditionally evaluated for mutations correlated to sites of carcinogenicity for the chemicals tested. 28 refs., 2 tabs.

OSTI ID:
468352
Journal Information:
Environmental and Molecular Mutagenesis, Journal Name: Environmental and Molecular Mutagenesis Journal Issue: 4 Vol. 28; ISSN 0893-6692; ISSN EMMUEG
Country of Publication:
United States
Language:
English

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