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PSEUDOMONAS AERUGINOSA INFECTION AND RADIOBIOLOGICAL RESEARCH AT ARGONNE NATIONAL LABORATORY: EFFECTS, DIAGNOSIS, EPIZOOTIOLOGY, CONTROL

Journal Article · · Lab. Animal Care
OSTI ID:4679987

Pseudomonas aeruginosa infection reduced the survival time of lethally irradiated mice from 13.8 days to 6.0 days. Many radiobiological studies were affected, partioularly those concerned with protection against radiation injury. Various diagnostic procedures were studied and evaluated. Tests based on P. aeruginosa's unique ability to produce pyocyanin were found to be the most useful for the mass screening of mouse colonies. The moat efficient technique consisted of culturing individual fecal or group water bottle samples in Wensinck's glycerol broth at 37 deg C for seven days and then testing the culture for the chloroform-soluble pigment. The epizootiology of the infection was also studied. One of 12 animal caretakers and about 26% of the cockroaches infesting the animal quarters were found to be infected, but an extensive survey of various inanimate materials (building, equipment, feeds, bedding) showed that when good sanitation is employed such materials are rarely infected. Control procedures based on the removal of the probable source of infection (animate vectors) and the prevention of the spread of existing infection through good sanitation are described. (auth)

Research Organization:
Argonne National Lab., Tenn.
NSA Number:
NSA-17-030236
OSTI ID:
4679987
Report Number(s):
TID-17601
Journal Information:
Lab. Animal Care, Journal Name: Lab. Animal Care Vol. Vol: 13
Country of Publication:
Country unknown/Code not available
Language:
English

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