A STUDY IN THE CONTROL OF PSEUDOMONAS AERUGINOSA IN MICE BY COLISTIN SULFATE
A specific-pathogen-free (SPF) colony of mice was developed by fostering cesarean-derived CF#1 mice on SPFCFN rats. Fecal cultures showed none of the mice to be positive for Salmonella, or any other organisms considered to be pathogens. There was, however, a hlgh incidence of P. aeruginosa, an organism usually present in a low incidence in conventional CF#1 mice. Because the mice appeared healthy, it was assumed that P. aeruginosa in the intestinal tract was not necessarily detrimental and that the unusual flora was perhaps a phenomenon of SPF development. After production of SPF-CF#1 mice had proceeded for some months, however, marked differences were noted in the response of these mice to radiation. X-ray doses that would kill conventional CF#1 mice in 48 to 72 hr killed the SPF stock in only a few hours. These findings were associated with the high incidence of P. aeruginosa and led to studies of ways to control, or possibly eliminate, this organism. Colistin sulfate in the drinking water significantly reduced the incidence of P. aeruginosa in these mice, as judged by cultures of intestinal contents. The reduced incidence was successfully maintained for a 20week period. Irradiation at the end of this time, in most instances, confirmed the bacteriological findings. The percentages of infected control and colistin-treated CF#1 mice were the same before and after 800-r whole- body x irradiation. (BBB)
- Research Organization:
- Carworth, Inc., New City, N.Y.
- NSA Number:
- NSA-17-035380
- OSTI ID:
- 4647589
- Journal Information:
- Lab. Animal Care, Journal Name: Lab. Animal Care Vol. Vol: 13
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
Similar Records
THE ORIGIN OF INDUCED PSEUDOMONAS AERUGINOSA BACTEREMIA IN IRRADIATED MICE
PSEUDOMONAS AERUGINOSA INFECTION AND ITS CONTROL IN THE RADIOBIOLOGICAL RESEARCH PROGRAM AT OAK RIDGE NATIONAL LABORATORY