HISTORADIOAUTOGRAPHIC STUDY OF THE INCORPORATION OF TRITIATED URIDINE IN TISSUE CULTURES OF NORMAL FIBROBLASTS OR THOSE SUBMITTED TO THE ACTION OF ANTIMITOTIC SUBSTANCES (in French)
Journal Article
·
· Arch. Biol. (Liege)
OSTI ID:4670615
The incorporation of uridine-H/sup 3/ was studied in chlck fibroblasts cultivated in vitro by a hanging drop technique. Control cultures were treated with ribonuclease or neutral deoxyribonuclease to investigate the metabolism and synthesis of RNA in the fibroblasts, either normal or treated by some antimitotic substances, including Be. In normal fibroblasts, within a few minutes, of uridine-H/sup 3/ addition, the nucleus, mainly the nucleolus, appears to be radioactive. A part of the newly synthesized RNA of the nucleolus is transferred to cytoplasm. After a long period of contact with uridine, the nucleoli are very strongly labeled (RNA), the rest of the nucleus only moderately (RNA and DNA), and the cytoplasm heavily (RNA). Thus, these studies showed that active synthesis of RNA takes place inside the nulcleus, and any synthesis eventually occurring in the cytoplasm is relatively weak or slow, indicating that at least a part of the cytoplasmic RNA originates from the nucleus. The synthesis of RNA continues during the preprophasic period of DNA synthesis and during the postsynthesis period. Beryllium, added at concentrations of 0.0025 to 0.00125M, inhibited incorporation of uridine into nucleus, particularly into nucleolus. This was noted at 4 and 24 hr after addition Be; no difference was noted in the effects of the two concentrations tested. Ribonuclease, added to living cultures, reduces the amount of tritiated uridine in the nucleus and cytoplasm. Trihydroxy-N-methylindole has no effect in this respect. (BBB)
- Research Organization:
- Originating Research Org. not identified
- NSA Number:
- NSA-17-035412
- OSTI ID:
- 4670615
- Journal Information:
- Arch. Biol. (Liege), Journal Name: Arch. Biol. (Liege) Vol. Vol: 72
- Country of Publication:
- Country unknown/Code not available
- Language:
- French
Similar Records
Electron microscopic examination of the sites of nuclear RNA synthesis during amphibian embryogenesis
Autoradiographic study of the nucleolar RNA metabolism during the oogenesis of Lineus ruber Mueller (Heteronemertes)
A COMPARISON OF MAMMALIAN CELL-KILLING BY INCORPORATED $sup 3$H-THYMIDINE AND $sup 3$H-URIDINE
Journal Article
·
Tue Aug 31 20:00:00 EDT 1965
· Journal of Cell Biology
·
OSTI ID:1629496
Autoradiographic study of the nucleolar RNA metabolism during the oogenesis of Lineus ruber Mueller (Heteronemertes)
Journal Article
·
Mon May 26 00:00:00 EDT 1975
· C. R. Hebd. Seances Acad. Sci., Ser. D, v. 280, no. 20, pp. 2365- 2368
·
OSTI ID:4110517
A COMPARISON OF MAMMALIAN CELL-KILLING BY INCORPORATED $sup 3$H-THYMIDINE AND $sup 3$H-URIDINE
Journal Article
·
Sun Sep 01 00:00:00 EDT 1963
· Intern. J. Radiation Biol.
·
OSTI ID:4037269