THE ROLES OF CELLULAR DIVISION AND MATURATION IN THE FORMATION OF PRECIPITATING ANTIBODY
Journal Article
·
· Journal of Immunology (U.S.)
OSTI ID:4669040
The relative importance of somatic division and conversion of pyronophilic cells into mature plasma cells, in the secondary antibody response, was studied in the lymphoid cells of rabbits immunized with bovine serum albumin (BSA). The anti-BSA response by isolated cells was investigated with the use of tritiated thymidine as a marker in combination with the immunofluorescent technique and colchicine, a mitotic inhibitor. The cells were cultured in diffusion chambers implanted into x-irradiated rabbits and mice. The results showed that not only were antibodycontaining cells dividing but that they were dividing at a rate significantly higher than that of the incompetent cells during the log phase of activity. The mean generation time was 12 hr during the log phase and 24 hr in the stationary phase. A direct correspondence was found between the rise in percentage of antibody-containing cells and rise in antibody titer of culture chamber fluid. All competent cells of the stationary phase were derived from precursor cells through somatic division. Cytomorphologic examinations of antibody-containing cells at intervals after antigenic stimulation suggest a gradual transition in the number and distribution of sites of antibody synthesis. It is speculated that these processes result from primary or secondary action of antigen upon the cell nucleus, and that the messenger RNA is first utilized by the ribosomes of the nucleolus. (BBB)
- Research Organization:
- Oak Ridge National Lab., Tenn.
- NSA Number:
- NSA-17-035519
- OSTI ID:
- 4669040
- Journal Information:
- Journal of Immunology (U.S.), Journal Name: Journal of Immunology (U.S.) Vol. Vol: 90; ISSN JOIMA
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
Similar Records
MITOTIC ACTIVITY OF IMMUNOLOGICALLY COMPETENT LYMPHOID CELLS TRANSFERRED INTO X-IRRADIATED RECIPIENTS
THE EFFECT OF X-IRRADIATION AND PASSIVE ANTIBODY ON IMMUNOLOGIC TOLERANCE IN THE RABBIT TO BOVINE SERUM ALBUMIN
ANTIBODY PRODUCTION BY IMMUNOLOGICALLY COMPETENT CELLS TRANSFERRED TO A HETEROLOGOUS HOST
Journal Article
·
Fri Jun 01 00:00:00 EDT 1962
· Laboratory Investigation (U.S.)
·
OSTI ID:4657591
THE EFFECT OF X-IRRADIATION AND PASSIVE ANTIBODY ON IMMUNOLOGIC TOLERANCE IN THE RABBIT TO BOVINE SERUM ALBUMIN
Journal Article
·
Tue Dec 31 23:00:00 EST 1963
· Journal of Immunology (U.S.)
·
OSTI ID:4069841
ANTIBODY PRODUCTION BY IMMUNOLOGICALLY COMPETENT CELLS TRANSFERRED TO A HETEROLOGOUS HOST
Journal Article
·
Thu Jan 31 23:00:00 EST 1963
· Laboratory Investigation (U.S.)
·
OSTI ID:4718956