Bacterial spore detection and determination by use of terbium dipicolinate photoluminescence
Journal Article
·
· Analytical Chemistry (Washington)
- Army Research Lab., Adelphi, MD (United States)
- Duke Univ., Durham, NC (United States)
A new method to detect bacterial endospores and determine their concentration was demonstrated by the addition of a solution of terbium chloride to a suspension of bacterial endospores. The terbium chloride reacted with the calcium dipicolinate in the spore case to form terbium(III) dipicolinate anion. Solid particles, including residual bacterial particles, were removed by filtering. The photoluminescence from the solution was measured as a function of excitation wavelength, emission wavelength, and bacterial endospore concentration. The photoluminescence from terbium(III) dipicolinate anion in the solution was easily identified. 15 refs., 5 figs.
- OSTI ID:
- 466367
- Journal Information:
- Analytical Chemistry (Washington), Journal Name: Analytical Chemistry (Washington) Journal Issue: 6 Vol. 69; ISSN ANCHAM; ISSN 0003-2700
- Country of Publication:
- United States
- Language:
- English
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