Characterization and genomic mapping of genes and pseudogenes of a new human protein tyrosine phosphatase

Journal Article · Mon Jul 01 04:00:00 EDT 1996 · Genomics

DOI:https://doi.org/10.1006/geno.1996.0336· OSTI ID:466032

Zhao, Zhaoyang; Lee, Cheng-Chi; Monckton, D G ^[1]

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1. Baylor College of Medicine, Houston, TX (United States); and others

Previously described protein tyrosine phosphatases (PTPs) are classified into three types according to their sequence homology and structural features. Here we describe the characterization of genes and pseudogenes of a member of a fourth type of PTP, designated protein tyrosine phosphatase 4A (PTP4A). The 167-amino-acid human PTPs, but does not show any other sequence homology to any of the previously described PTPs. Two cDNA encoding PTP4A that differed in their noncoding regions were isolated. Another cDNA that has a high level of sequence identity with these two cDNAs and a deletion in the coding region was also isolated. Northern analysis using a probe from a common 3{prime}-untranslated region of the cDNAs recognized mRNAs of about 2 and 4 kb. Both species of mRNA were seen in all human adult and fetal tissues tested. Fluorescence in situ hybridization mapping of the corresponding yeast artificial chromosome clones and sequence-tagged site analysis suggested that one of the PTP4A coding genes is located at 1p35 and the other is on chromosome 11. A processed pseudogene for PTP4A was found in the BRCA1 region of 17q21 and shares 96% sequence identity to one of the PTP4A coding cDNAs. Our studies also suggest the existence of another processed pseudogene on chromosome 11. 31 refs., 6 figs., 1 tab.

• OSTI ID: 466032
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 1 Vol. 35; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
AMINO ACIDS
ANIMAL CELLS
CELL DIFFERENTIATION
DNA SEQUENCING
DNA-CLONING
FLUORESCENCE
GENE MUTATIONS
GENE REGULATION
GENETIC MAPPING
GROWTH
HUMAN CHROMOSOME 1
HUMAN CHROMOSOME 17
HUMAN CHROMOSOMES
IN-SITU HYBRIDIZATION
MESSENGER-RNA
METABOLISM
MORPHOLOGY
ONCOGENIC TRANSFORMATIONS
PHOSPHATASES
PHOSPHORYLATION
POLYMERASE CHAIN REACTION
PROBES
PROTEINS
STRUCTURE-ACTIVITY RELATIONSHIPS
TISSUE DISTRIBUTION
TYROSINE