Physical mapping of the Bloom syndrome region by the identification of YAC and P1 clones from human chromosome 15 band q26.1

Journal Article · Mon Jul 01 04:00:00 EDT 1996 · Genomics

DOI:https://doi.org/10.1006/geno.1996.0330· OSTI ID:466026

Straughen, J; Groden, J ^[1]; Ciocci, S ^[2]

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1. Univ. of Cincinnati College of Medicine, OH (United States)
2. New York Blood Center, NY (United States); and others

The gene for Bloom syndrome (BLM) has been mapped to human chromosome 15 band q26.1 by homozygosity mapping. Further refinement of the location of BLM has relied upon linkage-disequilibrium mapping and somatic intragenic recombination. In combination with these mapping approaches and to identify novel DNA markers and probes for the BLM candidate region, a contiguous representation of the 2-Mb region that contains the BLM gene was generated and is presented here. YAC and P1 clones from the region have been identified and ordered by using previously available genetic markers in the region along with newly developed sequence-tagged sites from radiation-restriction map of the 2-Mb region that allowed estimation of the distance between polymorphic microsatellite loci is also reported. This map and the DNA markers derived from it were instrumental in the recent identification of the BLM gene. 25 refs., 3 figs., 3 tabs.

• OSTI ID: 466026
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 1 Vol. 35; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
BIOLOGICAL MARKERS
CHROMOSOMAL ABERRATIONS
CONTIGS
DNA SEQUENCING
DNA-CLONING
FLUORESCENCE
GENE MUTATIONS
GENE RECOMBINATION
GENES
GENETIC MAPPING
HEREDITARY DISEASES
HUMAN CHROMOSOME 15
HYBRIDIZATION
IN-SITU HYBRIDIZATION
MAN
NUCLEOTIDES
POLYMERASE CHAIN REACTION
PROBES
RADIATION INDUCED MUTANTS
RECESSIVE MUTATIONS
RESOLUTION
RFLPS
SOMATIC CELLS
SOMATIC MUTATIONS
STERILITY