Comparative analysis of a conserved zinc finger gene cluster on human chromosome 19q and mouse chromosome 7

Journal Article · Mon Apr 01 04:00:00 EST 1996 · Genomics

DOI:https://doi.org/10.1006/geno.1996.0166· OSTI ID:465931

Shannon, M; Mucenski, M L; Stubbs, L ^[1]

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1. Oak Ridge National Lab., TN (United States); and others

Several lines of evidence now suggest that many of the zinc-finger-containing (ZNF) genes in the human genome are arranged in clusters. However, little is known about the structure or function of the clusters or about their conservation throughout evolution. Here, we report the analysis of a conserved ZNF gene cluster located in human chromosome 19q13.2 and mouse chromosome 7. Our results indicate that the human cluster consists of at least 10 related Kruppel-associated box (KRAB)-containing ZNF genes organized in tandem over a distance of 350-450 kb. Two cDNA clones representing genes in the murine cluster have been studied in detail. The KRAB A domains of these genes are nearly identical and are highly similar to human 19q13.2-derived KRAB sequences, but DNA-binding ZNF domains and other portions of the genes differ considerably. The two murine genes display distinct expression patterns, but are coexpressed in some adult tissues. These studies pave the way for a systematic analysis of the evolution of structure and function of genes within the numerous clustered ZNF families located on human chromosome 19 and elsewhere in the human and mouse genomes. 32 refs., 7 figs.

• DOE Contract Number: AC05-84OR21400; W-7405-ENG-48
• OSTI ID: 465931
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 1 Vol. 33; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
AMINO ACID SEQUENCE
ANIMAL CELLS
BIOLOGICAL EVOLUTION
CELL DIFFERENTIATION
CELL PROLIFERATION
CHROMOSOMES
COSMIDS
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
G CODES
GENE REGULATION
GENES
GENETIC MAPPING
HUMAN CHROMOSOME 19
MICE
ONTOGENESIS
PROTEINS
STRUCTURE-ACTIVITY RELATIONSHIPS