POLAROGRAPHIC AND ELECTROPHORETIC STUDIES ON THE EFFECT OF A NEUTRON FLUX ON SOLUTIONS OF SERUM PROTEINS

Journal Article · Sun Jan 01 00:00:00 EST 1961 · Acad. Rep. Populare Romine, Studii Cercetari Biochim.

OSTI ID:4645522

Buruiana, L M; Pavlu, V; Dema, I; Diaconescu, C; Dema, A

>The albumins and globulins in bovine, equine, porcine, and avian serums were separated by precipitation with half-saturated ammonium sulfate solution. The separated proteins were dissolved in 0.05 M phosphate buffer at pH 6.8 and dialyzed for 24 hr. In this form they were exposed for 1 hr in a nuclear reactor (of the Institute of Atomic Physics, Bucharest) with a flux consisting of thermal neutrons (2.3 x 10/sup 7//cm/sup 2//sec), epithermal neutrons (7.1 x 10/sup 5// cm/sup 2//sec), fast neutrons (4.0 x 10/sup 7//cm/sup 2//sec), and gamma radiation (0.06 r/sec). Changes in the polarographic behavior of the proteins, in the presence of Co ion and over the range -1.2 to --1.9 v, and in electrophoretic mobility, on filter paper at pH 8.6 and ionic strength 0.05 Veronal buffer, were examined following irradiation. Irradiation caused a lowering of the amplitude of the polarographic half-wave, at protein concentrations from 0.36 to 2.88 mg/100 mi, and a shift in the peak toward lower voltage values, indicating that irradiation alters the ability of the proteins to bind Co ions. These changes varied with the protein concentration, and for the protein fractions and species examined, avian globulins showed the most marked polarographically demonstrable changes following irradiation. No changes in the electrophoretic mobilities of the various globulins were noted after exposure, and of the albumins, only those of the horse and bird showed increased mobilities postirradiation. It was concluded that irradiation of proteins in a neutron flux produces irreversible changes on the surface groups on the molecules, which are reflected by their ability to bind ions and by their electrostatic charges. (BBB)

• Research Organization: Facultatea de Medicina Veterinara; and Institutul de Fizica Atomica, Bucharest
• NSA Number: NSA-17-038752
• OSTI ID: 4645522
• Journal Information: Acad. Rep. Populare Romine, Studii Cercetari Biochim., Vol. Vol: 4; Other Information: Orig. Receipt Date: 31-DEC-63
• Country of Publication: Country unknown/Code not available
• Language: Rumanian

Related Subjects

BIOLOGY AND MEDICINE
ACIDITY
ALBUMINS
AMMONIUM COMPOUNDS
ANIMALS
BIRDS
BLOOD SERUM
CATTLE
COBALT
DIALYSIS
DIFFUSION
ELECTRIC CHARGES
ELECTRIC POTENTIAL
ELECTROPHORESIS
EPITHERMAL NEUTRONS
FAST NEUTRONS
GAMMA RADIATION
GLOBULINS
HORSES
IONS
MEMBRANES
MOLECULES
NEUTRON BEAMS
PHOSPHATES
POLAROGRAPHY
PRECIPITATION
PROTEINS
QUANTITY RATIO
RADIATION EFFECTS
REACTORS
SEPARATION PROCESSES
SOLUTIONS
SULFATES
SURFACES
SWINE
THERMAL NEUTRONS