Low-Voltage Electron Beam Irradiation of Normal and 5-Bromouridine Deoxyriboside-Treated L-P59 Mouse Fibroblast Cells In Vitro
Low-voltage electron beams were used to irradiate to selective depths within mouse fibroblast cells grown in tissue culture, with and without the addition of 5-bromouridine desoxyriboside (BUdR) in an investigation of the role of nuclear and chromosomal damage in radiation killing of mammalian cells. The electron beam irradiation system is described and problems of dosage measurement are discussed. Representative plots of the survival data indicate that two mechanisms may be involved in the radiation killing of mammalian cells, one of which involves chromatin located on or near the nuclear membrane and is potentiated by BUdR directly associated with desoxyribonucleic acid (DNA) and another that does not involve DNA. (C.H.)
- Research Organization:
- Univ. of Texas, Houston
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-17-035538
- OSTI ID:
- 4645264
- Journal Information:
- Nature (London), Journal Name: Nature (London) Journal Issue: 4895 Vol. 199; ISSN 0028-0836
- Publisher:
- Nature Publishing Group
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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