Title: PRODUCTION OF A NERVOUS-SYSTEM-SPECIFIC PROTEIN (14-3-2) BY HUMAN NEUROBLASTOMA CELLS IN CULTURE.

In order to test the generality of radiofrequency-radiation-induced change in alternation of /sup 45/Ca/sup 2/plus// efflux from avian and feline brain tissues, human neuroblastoma cells were exposed to electromagnetic radiation at 147 MHz, amplitude modulated (AM) at 16 Hz, at specific absorption rates (SAR) of 0.1, 0.05, 0.01, 0.005, 0.001, and 0.0005 Wkg. Significant /sup 45/Ca/sup 2/plus// efflux was obtained at SAR values of 0.05 and 0.005 Wkg. Enchanced efflux at 0.05 Wkg peaked at the 13-to-16 Hz and at the 57.5-to-60 Hz modulation ranges. A Chinese hamster-mouse hybrid neuroblastoma was also shown to exhibit enchanced radiation-induced /sup 45/Ca/sup 2/plus//more » efflux at an SAR of 0.05 Wkg, using 147 MHz, AM at 16 hz. These results confirm that amplitude-modulated radiofrequency radiation can induce response in cells of nervous tissue origin from widely different animal species including humans. The results are also consistent with reports of similar findings in avian and feline brain tissue reported by others and indicate the general nature of the phenomenon. 9 refs., 3 tabs.« less

Radiolabeled /sup 14/C soman, a potent anticholinesterase, was administered to rats at a dose of 0.75 LD50 (17.3 micrograms/kg, i.m.). Prior to being quick frozen, the animals were held for 2 min, 32 min or 48 h after dosing. Analysis of autoradiographs taken from cryostat sections through the central nervous system (CNS) showed that at 2 and 32 min radiolabel was primarily distributed in the blood, the choroid plexus and the cerebrospinal fluid (CSF), with several brain nuclei showing small increases in labeling density. At 48 h there was marked accumulation of radiolabel in the caudate and accumbens nuclei comparedmore » to all other brain areas. The above results showed little similarity between cholinesterase localization and distribution of radiolabel.« less

The monochromaticity of a ''single line'' neutron source is of utmost importance whenever secondary neutron yields over a wide energy range must be measured. Among the neutron sources considered, /sup 3/H(p,n)/sup 3/He is best if a suitable beam stop is used; for example, /sup 28/Si or /sup 58/Ni. Tantalum and gold give much larger backgrounds. Neutron emission spectra from the hydrogen gases and from various beam stops have been measured and are given in 1-MeV steps for primary neutron energies between 10 and 14 MeV.