PURIFICATION OF RADIOACTIVE FOOT-AND-MOUTH DISEASE VIRUS
Investigations on the purification of small quantities of radioactively labeled foot-and-mouth disease (FMD) virus are described. The results indicated that there is present in untreated virus suspensions and pellets a complex of the 7-m mu component and cellular material, which has the same sedimentation characteristics in sucrose density gradients as the 25-m mu infective component but is disrupted by deoxycholate into smaller components that sediment much more slowly in the sucrose gradient. The ratio of plaque-forming units and complement- fixing actlvity to radioactive counts in the individual fractions in sucrose gradients of deoxycholate-treated pellets was remarkably constant, indicating that the material in the peak was homogeneous. The experiments provided substantial evidence that the virus obtained from deoxycholate-treated pellets is free from substances found in uninfected cells. (P.C.H.)
- Research Organization:
- Research Inst. (Animal Virus Diseases), Pirbright, Surrey, Eng.
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-17-038673
- OSTI ID:
- 4634840
- Journal Information:
- Nature, Journal Name: Nature Vol. Vol: 199
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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