EFFECTS OF WHOLE-BODY Co$sup 60$ IRRADIATION ON THE ELIMINATION OF AN I$sup 131$-LABELED ANTIGEN. I. STUDY OF THE SENSITIZATION PHENOMENON

Journal Article · Thu Nov 01 04:00:00 EST 1962 · Minerva Fisioterap.

OSTI ID:4629003

Piccotti, F; Sannazzari, G L; Torretta, A

Adult rabbits were sensitized by intradermal injection of 10 mu g human serum albumin (HSA), and in some cases exposed 12 hr later to 500-r gamma radiation. In other animals irradiation preceded injection of antigen by 12 hr, some received radiation only, and others no treatment. Eight days later I/sup 131/-labeled HSA was injected intravenously and its rate of elimination from the circulation followed by serial assays of blood radioactivity. In rabbits receiving no treatments, HSA-I/sup 131/ in blood fell to 14% of the initial level and then, at a more rapid rate, to 0.2% by the 7th day. Faster (immune) elimination of antigen was noted in the sensitized rabbits, only 1.2 and 0.14% remaining by the 4th and 5th days. Elimination of antigen was considerably slowed in rabbits receiving irradiation alone, with 18% remaining on the 4th and 0.14% on the 10th day, showing the inhibitory effect of irradiation on the nonimmune mechanlsms for removing foreign protein from the circulation. Rabbits irradiated 12 hr after sensitization cleared the antigen from the blood much faster (0.8 and 0.2% remaining at 4 and 5 days) than those irradiated before (12 and 6% at 4 and 5 days) and slightly faster than sensitized but nonirradiated rabbits. The results indicate that antibody is rapidly synthesized after introduction of antigen and that midlethal irradiation 12 hr later does not impair the immune mechanisms for removal of the antigen from the circulation when it is reintroduced 8 days later. In contrast, irradiation before sensitization abolishes the immune mechanism for elimination of antigen. (BBB)

• Research Organization: Universita, Turin
• NSA Number: NSA-17-038819
• OSTI ID: 4629003
• Journal Information: Minerva Fisioterap., Journal Name: Minerva Fisioterap. Vol. Vol: 7
• Country of Publication: Country unknown/Code not available
• Language: Italian

Related Subjects

AGE
ALBUMINS
ANTIBODIES
ANTIGENS
BIOLOGY AND MEDICINE
BLOOD CIRCULATION
BLOOD SERUM
COBALT 60
GAMMA RADIATION
IODINE 131
LABELLED COMPOUNDS
MAN
METABOLISM
RABBITS
RADIATION DOSES
RADIATION EFFECTS
RADIOACTIVITY