Altered enzyme expression in a population of creosote-resistant mummichog (Fundulus heteroclitus)
Conference
·
OSTI ID:460481
- Coll. of William and Mary, Gloucester Point, VA (United States). School of Marine Science
A population of mummichog in the Elizabeth River, Virginia inhabit a site severely contaminated with creosote. Although adult fish develop cancer, all life stages are resistant to the acute effects of creosote-contaminated sediments. Creosote is a complex mixture of PAHs, N,S,O-heterocyclics and phenols. The site is also contaminated with other organic contaminants and metals. Studies are underway to identify possible resistance mechanisms in this population. Cytochrome P4501A (CYP1A) is the major enzyme involved in activation of PAHs and also contributes to generation of reactive oxygen species (ROS). This enzyme is depressed in liver of resistant fish and unresponsive to CYP1A inducers. Glutathione S-transferases (GSTs) are major cytosolic proteins involved in diverse detoxification mechanisms. GST levels and activity are clearly elevated in the resistant population. Monoclonal antibody to purified mummichog GST recognizes a form that is elevated approximately six-fold in the resistant fish relative to reference fish. This form appears to be a major cytosolic protein in the resistance population. P-glycoprotein, an efflux pump for diverse xenobiotics, is abundantly expressed on the canalicular surface of hepatocytes and over expressed in advanced hepatic lesions. The pattern of enzyme expression observed in the resistant population is similar to that observed in multidrug-resistant cell lines.
- OSTI ID:
- 460481
- Report Number(s):
- CONF-961149--
- Country of Publication:
- United States
- Language:
- English
Similar Records
Altered expression of glutathione S-transferases in the liver of xenobiotic-resistant mummichog (Fundulus heteroclitus)
Hepatic neoplasms in the mummichog Fundulus heteroclitus from a creosote-contaminated site
Exocrine pancreatic neoplasms in the mummichog (fundulus heteroclitus) from a creosote-contaminated site
Conference
·
Sat Dec 30 23:00:00 EST 1995
·
OSTI ID:372521
Hepatic neoplasms in the mummichog Fundulus heteroclitus from a creosote-contaminated site
Journal Article
·
Sat Sep 15 00:00:00 EDT 1990
· Cancer Research; (USA)
·
OSTI ID:6371127
Exocrine pancreatic neoplasms in the mummichog (fundulus heteroclitus) from a creosote-contaminated site
Technical Report
·
Fri Dec 31 23:00:00 EST 1993
·
OSTI ID:6579527